WEKO3
アイテム
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タンザニア北東部産Rice yellow mottle virusの解析
https://nodai.repo.nii.ac.jp/records/584
https://nodai.repo.nii.ac.jp/records/584c10551cb-120d-46c5-ba63-fed3040209a1
名前 / ファイル | ライセンス | アクション |
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KJ00010061259.pdf (1.6 MB)
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Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2015-12-18 | |||||
タイトル | ||||||
タイトル | タンザニア北東部産Rice yellow mottle virusの解析 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Characterization of Rice yellow mottle virus in north-eastern Tanzania | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | イネ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 系統解析 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 血清型 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | タンザニア | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rice yellow mottle virus(RYMV) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Phylogenetic analysis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Rice | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Rice yellow mottle virus (RYMV) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Serotype | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Tanzania | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00164184 | |||||
論文名よみ | ||||||
タイトル | タンザニア ホクトウブ サン Rice yellow mottle virus ノ カイセキ | |||||
著者 |
鵜家, 綾香
× 鵜家, 綾香× Naswiru Tibanyendela× 夏秋 啓子× 関谷 信人× 大泉 暢章× Ayaka, Uke× Naswiru Tibanyendela× Keiko T. Natsuaki× Nobuhito Sekiya× Nobuaki Oizumi |
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著者所属(日) | ||||||
東京農業大学大学院農学研究科国際農業開発学専攻 | ||||||
著者所属(日) | ||||||
キリマンジャロ農業試験場,タンザニア | ||||||
著者所属(日) | ||||||
東京農業大学大学院農学研究科国際農業開発学専攻 | ||||||
著者所属(日) | ||||||
東京大学大学院農学生命科学研究科 | ||||||
著者所属(日) | ||||||
JICA国際協力機構 | ||||||
著者所属(英) | ||||||
en | ||||||
Department of International Agricultural Development, Graduate School of Agriculture, Tokyo University of Agriculture | ||||||
著者所属(英) | ||||||
en | ||||||
Kilimanjaro Agricultural Training Centre, Moshi, Tanzania | ||||||
著者所属(英) | ||||||
en | ||||||
Department of International Agricultural Development, Graduate School of Agriculture, Tokyo University of Agriculture | ||||||
著者所属(英) | ||||||
en | ||||||
Institute for Sustainable Agro-ecosystems Services, Graduate School of Agricultural and Life Sciences, The University of Tokyo | ||||||
著者所属(英) | ||||||
en | ||||||
Japan International Cooperation Agency | ||||||
記事種別(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 論文 | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | アフリカにおいて重要な穀物のひとつであるイネ(Oryza sativa L.)は,Rice yellow mottle virusによる重大な被害を受けており,タンザニアにも広がっている。水稲栽培の最も進んだ地域の一つであるローワーモシ灌漑地区を含む北東部で,ウイルス病様病徴の認められる灌漑水田において検体の採集を行った。採集した試料は,電子顕微鏡を用いたウイルス粒子の観察,DAS-ELISA法,PCR法による検出を行ったところ,RYMVの感染が確認された。3つの分離株Tz-12-20,Tz12-22,Tz10-36の PCR産物については,遺伝子解析を行い系統と血清型の判別を行った。その結果,S4型(Pinel et al., 2009)分離株であることが示され,また,系統解析から,ビクトリア湖周辺分離株であるS4-lvやS4-lm,S4-mgとは異なる単一のクラスターを形成し,新しいグループ(S4-mk)であると考えられた。さらに系統樹から,S4-mk分離株はケニアの西南地域からタンザニアの北東部において広がっている分離株であることを示した。系統樹において単一のクラスターを形成した一方,S4-mk分離株と他のS4型分離株(S4-lv,S4-lm,S4-mg)間のアミノ酸配列で,96%以上の高い相同性を示した。また,アミノ酸配列から血清型の判別が可能(Fargette et al., 2002)であることから,タンザニア産RYMVでこの手法を用いて血清型を検討したところ,類別可能な5種類の血清型のうち,Ser4に分類された。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Rice (Oryza sativa L.) is an important cereal-grain in some African countries and known to be attacked by Rice yellow mottle virus (RYMV). Rice plants with virus like symptoms were collected from lower Moshi and other areas in the north-eastern region of Tanzania. RYMV was detected by DAS-ELISA, RT-PCR and/or electron microscopy from almost all collected samples. Some RYMV isolates were sequenced and identified as strain S4. Also, three of them, Tz-12-20, Tz-12-22 and Tz-10-36, were clustered as a new group named S4-mk (Mt. Kilimanjaro). Cluster of S4-mk formed a monophyletic group of isolates in strain S4-lv reported from the Tanzanian side of the Lake Victoria. However, S4-mk and S4-lv lineages are separated from each other and S4-mk has the characteristic three amino acid substitutions. The isolates of S4-mk were also closely related to that of strain S4-mg. Amino acid sequences of the coat protein of the isolates in group S4-mk showed over 96% identity with those in other strain S4 varieties, S4-lv, S4-mg and S4-lm. The phylogenetic tree also indicated the possibility of strain S4-mk to have dispersed from south-western Kenya to north eastern Tanzania. As for the serotyping, followed by Fargette et al. (2002), these three isolates in S4-mk were classified into serotype 4. | |||||
書誌情報 | 巻 60, 号 3, p. 116-126 | |||||
レポート・講演番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | E | |||||
表示順 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 2 | |||||
アクセション番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | KJ00010061259 |