{"created":"2023-06-19T07:30:50.894610+00:00","id":912,"links":{},"metadata":{"_buckets":{"deposit":"8ea0a953-71ab-4b78-8987-80a64cf3f6c1"},"_deposit":{"created_by":3,"id":"912","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"912"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000912","sets":["47:48:199:200"]},"author_link":["4518","4517","4514","4515","4513","4516"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"52","bibliographicPageStart":"47","bibliographicVolumeNumber":"67","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"エゾユキウサギ（Lepus timidus ainu）はユキウサギの北海道固有亜種であり，肉食動物の餌資源として重要な生態学的役割を担うと考えられる。その一方で，その詳細な生態学的特性は明らかにされていない。それはエゾユキウサギが夜行性の単独行動であり，周囲の環境に隠れて生活しているためと推測される。本研究は，エゾユキウサギのZFXおよびZFY遺伝子におけるエキソン11の部分配列を決定し，BglIIおよびAluIによって雌雄を区別できる塩基置換部位を明らかにした。PCR-RFLPの結果，オスにおいてBglIIによって消化されたZFY由来のPCR産物と未消化のZFX由来の断片が検出され，メスでは未消化のバンドのみが確認された。以上の結果はこれまで報告された他のウサギ目と一致した。したがって，ZFX/ZFYを用いた雌雄鑑定はエゾユキウサギにおいても有用であることが確認された。一方，エゾユキウサギのZFYにはヨーロッパに生息するユキウサギ（L. timidus）との間に1塩基置換が検出された。加えて，本研究は非侵襲試料である糞由来DNAを用いた雌雄判定を試みた。その結果，成功率は約56%と低いものの，糞由来を用いたZFX/ZFYシステムによる雌雄鑑定は可能であった。加えて，冬季（2月）に採集された糞を用いた雌雄判定は，ほぼ100%の成功率であった。これらの結果は，フィールドに残された痕跡物，特に冬季に採集された糞を利用したエゾユキウサギのDNA分析が可能であることを示唆した。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The Ezo mountain hare (Lepus timidus ainu) is an endemic subspecies of Hokkaido, a northern island of Japan.  Although the Ezo mountain hare is an important member of the ecosystem as a prey species for predators, its detailed ecological features are still unknown because of the nocturnal and solitary behavior that makes them difficult to find.  In this study, we determined a partial sequence of 392-bp in exon 11 of the zinc finger protein X-linked gene (ZFX) and zinc finger protein Y-linked gene (ZFY) and detected the nucleotide substitution sites between the sexes.  In addition, a nucleotide substitution was identified in exon 11 of ZFY between the Ezo mountain hare and Lepus timidus.  In tissue-derived DNA, sex-specific band patterns were detected after digestion of polymerase chain reaction (PCR) products using the BglII restriction enzyme.  Therefore, the ZFX/ZFY marker may be useful for molecular sexing in the Ezo mountain hare.  Furthermore, sex could be distinguished in ～60% of the total tested feces-derived DNA.  Although a relatively low success rate of sexing was shown in the analysis using feces-derived DNA, our study showed that the ZFX/ZFY marker may be useful for molecular sexing of the Ezo mountain hares using feces obtained non-invasively.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"6","subitem_description_type":"Other"}]},"item_1_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"短報","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Note","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京農業大学大学院生物産業学研究科生物生産学専攻"},{"subitem_text_value":"東京農業大学大学院生物産業学研究科生物生産学専攻"},{"subitem_text_value":"東京農業大学大学院生物産業学研究科生物生産学専攻"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Graduate School of Bioindustry, Tokyo University of Agriculture"},{"subitem_text_language":"en","subitem_text_value":"Graduate School of Bioindustry, Tokyo University of Agriculture"},{"subitem_text_language":"en","subitem_text_value":"Graduate School of Bioindustry, Tokyo University of Agriculture"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"エゾユキウサギ　フン　ユライ　DNA　ヲ　モチイタ　ZFX/ZFY　イデンシ　ニ　ヨル　シユウ　ノ　DNA　カンテイ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高橋, 亜希"},{"creatorName":"タカハシ, アキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"草野, 奈央"},{"creatorName":"クサノ, ナオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"和田, 健太"},{"creatorName":"ワダ, ケンタ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Aki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kusano, Nao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4517","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wada, Kenta","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4518","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-27"}],"displaytype":"detail","filename":"67_47.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"67_47","url":"https://nodai.repo.nii.ac.jp/record/912/files/67_47.pdf"},"version_id":"532b7cee-aaf7-4a77-895f-6c5a506bd7f8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"エゾユキウサギ","subitem_subject_scheme":"Other"},{"subitem_subject":"糞由来DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"雌雄鑑定","subitem_subject_scheme":"Other"},{"subitem_subject":"ZFX/ZFY遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"Ezo mountain hare","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"feces-derived DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"molecular sexing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ZFX/ZFY","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"エゾユキウサギ糞由来DNAを用いたZFX/ZFY遺伝子による雌雄のDNA鑑定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エゾユキウサギ糞由来DNAを用いたZFX/ZFY遺伝子による雌雄のDNA鑑定"},{"subitem_title":"Molecular Sexing of the Ezo Mountain Hare (Lepus timidus ainu) based on ZFX/ZFY Genes and Application for Using Feces-Derived DNA","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["200"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-06-27"},"publish_date":"2022-06-27","publish_status":"0","recid":"912","relation_version_is_last":true,"title":["エゾユキウサギ糞由来DNAを用いたZFX/ZFY遺伝子による雌雄のDNA鑑定"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:39:39.528747+00:00"}