{"created":"2023-06-19T07:30:47.633127+00:00","id":829,"links":{},"metadata":{"_buckets":{"deposit":"e9391e62-0c1d-4067-8a2e-e8b0ae88030d"},"_deposit":{"created_by":3,"id":"829","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"829"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000829","sets":["47:48:187:188"]},"author_link":["4251","4250","4247","4248","4252","4249"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"38","bibliographicPageStart":"33","bibliographicVolumeNumber":"65","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"近年では，糞，毛，羽などから遺伝情報が得られるようになり，銃やボーガンを使用して，遠距離から非直接的なサンプルが取れるようにもなった。しかし，このようなサンプルは，同一個体から複数回サンプリングしてしまう可能性があるため，遺伝子による個体識別が必要不可欠である。遺伝子を利用した個体識別は，一般的に複数のマイクロサテライト遺伝子座を使って行われる。個体識別に必要な遺伝子座とその数は，事前に既知のデータから遺伝子座ごとに重複率（PID : probability of identity）PID（obs）を算出して決定しておくことが望ましい。しかし，サンプル数が多く手に入らない場合，得られたサンプルの範囲内で予測値を推定する方法がある。PIDの予測値には，ハーディー・ワインベルグ平衡下の集団で血縁のない個体間の重複率（PID（theo））と，兄弟関係にある個体間の重複率（PID（sib））があり，実際の集団の重複率は，PID（theo）を下限，PID（sib）を上限と2つの予測値の間に位置するため，多くの動物では，PID（sib）の利用が推奨されている。本研究では，先行研究で集団が分かれることが示唆されているえりもと道東のハーバーシール2集団で，別個体と判明しているサンプルを基にPID（obs）と，2つの予測値を比較し，個体識別に必要な遺伝子座および遺伝子座数，予測値を算出するのに必要なサンプル数を決定した。その結果，日本のハーバーシールのPID（obs）は，PID（theo）とほぼ同等の値であり，4遺伝子座の使用で個体識別が可能であることを示した。一方で，個体識別に適した遺伝子座は，えりもと道東で異なった。さらに，日本のハーバーシールでは，20サンプルからPID（theo）を算出すれば，個体識別に有用な遺伝子座数の決定が行えることが示唆された。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"In recent years, genetic information has became much easier to obtain from feaces, hairs and feathers, and samples can be taken remotely by using biopsy darts fitted in guns and bowgun.  Individual identification based on genetic information is crucial when using these non-distructive samples to avoid duplicate collection from single individuals, which may easily happen.  In general, multiple microsatellite loci are used for individual identification.  It is preferable that combination and the number of loci used for the analysis are decided using known individuals by calculating the proportion of all possible pairs of individuals that have the same genotypes (PID : probability of identity).  However, if one does not have enough number of samples beforehand, theoretical estimator can be calculated using available samples.  Theoretical PID assumes the population is in Hardy-Weinberg equilibrium and there are equations for unrelated individuals (PID(theo)) and siblings (PID(sib)).  Since the observed PID generally lies between PID(theo) (lower boundary) and PID(sib) (upper boundary), it is recommended to use PID(sib) in many terrestrial animals.  In this study, we calculated observed PID (PID(obs)) and two theoretical PIDs for two genetically distinct populations of Japanese harbour seals, Erimo and eastern Hokkaido, using the samples that are known to be from different individuals.  Three PIDs are then compared, and combinations and numbers of loci essential for individual identification were investigated.  The number of samples required for estimating PID are also investigated by picking different numbers of samples randomly for 100 times.  The results of estimation indicated that PID(obs) for Japanese harbour seals showed similar values to PID(theo), and that at least 4 loci are required for identification.  On the other hand, the loci useful for individual identification in Erimo and Eastern Hokkaido differed.  Furthermore, it was suggested that 20 samples are enough to estimate PID(theo) and to detect loci useful for individual identification.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"5","subitem_description_type":"Other"}]},"item_1_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"短報","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Note","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京農業大学大学院生物産業学研究科生物産業学専攻"},{"subitem_text_value":"東京農業大学大学院生物産業学研究科生物産業学専攻，NPO法人北の海の動物センター"},{"subitem_text_value":"東京農業大学農学部生物資源開発学科"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Graduate School of Bio-Industry, Tokyo University of Agriculture"},{"subitem_text_language":"en","subitem_text_value":"Graduate School of Bio-Industry, Tokyo University of Agriculture, NPO Marine Wildlife Center of JAPAN"},{"subitem_text_language":"en","subitem_text_value":"Department of Bioresource Development, Faculty of Agriculture, Tokyo University of Agriculture"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"マイクロサテライト　イデンシ　ザ　ヲ　リヨウ　シタ　ニホンサン　ハーバーシール　ノ　コタイ　シキベツホウ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"水野, 米利子"},{"creatorName":"ミズノ, マリコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小林, 万里"},{"creatorName":"コバヤシ, マリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4248","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木, 剛"},{"creatorName":"ササキ, タケシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4249","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizuno, Mariko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4250","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Mari","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4251","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasaki, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4252","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-31"}],"displaytype":"detail","filename":"65_33.pdf","filesize":[{"value":"674.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"65_33","url":"https://nodai.repo.nii.ac.jp/record/829/files/65_33.pdf"},"version_id":"429458a9-85d7-462c-91a6-17e2a28cb9f0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"個体識別","subitem_subject_scheme":"Other"},{"subitem_subject":"PID","subitem_subject_scheme":"Other"},{"subitem_subject":"日本産ハーバーシール","subitem_subject_scheme":"Other"},{"subitem_subject":"ゼニガタアザラシ","subitem_subject_scheme":"Other"},{"subitem_subject":"Phoca vitulina stejnegeri","subitem_subject_scheme":"Other"},{"subitem_subject":"individual identification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PID","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Japanese harbour seals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Kuril harbour seals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phoca vitulina stejnegeri","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マイクロサテライト遺伝子座を利用した日本産ハーバーシールの個体識別法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マイクロサテライト遺伝子座を利用した日本産ハーバーシールの個体識別法"},{"subitem_title":"Individual Identification of Japanese Harbour Seals Using Nuclear Microsatellite loci","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["188"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-31"},"publish_date":"2020-07-31","publish_status":"0","recid":"829","relation_version_is_last":true,"title":["マイクロサテライト遺伝子座を利用した日本産ハーバーシールの個体識別法"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:43:41.715923+00:00"}