{"created":"2023-06-19T07:30:45.868127+00:00","id":786,"links":{},"metadata":{"_buckets":{"deposit":"6650fdc8-2e84-47e5-8778-9dfebd9ffe0b"},"_deposit":{"created_by":3,"id":"786","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"786"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000786","sets":["47:48:181:182"]},"author_link":["4085","4086"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"31","bibliographicPageStart":"29","bibliographicVolumeNumber":"64","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"農作物の育種現場でDNAマーカーを利用した個体選抜を行うには，PCRに必要なゲノムを多検体から迅速に抽出する必要がある。検体数が少ない場合には実績ある市販のゲノム抽出用キットを利用すればPCRに用いるゲノムを確実に抽出できるが，検体数が多い場合には実験労力やコストが問題となるため，多検体から簡易にゲノムを抽出できる方法が必要である。そこで，DNAが二酸化ケイ素に結合しやすい性質を利用して，より簡易なゲノム抽出の方法を検討した。供試植物には，ゲノムを抽出するのが難しい植物として知られているレタスを用いた。その結果，ガラス繊維濾紙をピペットチップ内に入れてゲノムの粗抽出液および2種類の洗浄液をそれぞれ数回ピペッティング操作するだけで，簡易にゲノムを抽出できることが確認できた。また，この方法で抽出したゲノムを用いてPCRを行ったところ，増幅産物を安定して得られることが明らかとなった。本研究により，PCRに用いるレタスのゲノムを簡易に抽出し，安定的にPCR産物が得られる方法を確立した。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"To use DNA markers for plant breeding, it is necessary to quickly extract genomes for PCR from multiple samples.  If the number of samples is small, it is possible to reliably extract the genome to be used for PCR by using a proven commercial genomic extraction kit, but when the number of samples is large, experiment labor and cost will be a problem.  By applying the glass fiber filter paper method which can extract the genome by utilizing the property that DNA is easy to bind to silicon dioxide, a simpler method of genome extraction was examined.  Lettuce, from which it is difficult to extract the genome, was used for the test crop.  As a result, it was confirmed that genome can be quickly extracted from multiple samples by placing the glass fiber filter paper in a pipette tip and pipetting the crude extract of genome and the two washing solutions several times respectively.  PCR using genomes extracted by this method revealed that amplified products can be stably obtained using KOD FX.  In this study, I established a method to rapidly extract the genome of lettuce used for PCR and stably obtain PCR products.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"4","subitem_description_type":"Other"}]},"item_1_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"短報","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Note","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"長野県野菜花き試験場　育種部"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Nagano Prefecture Vegetable and Ornamental Crops Experiment Station"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"DNAマーカーセンバツ　ニ　リヨウカノウ　ナ　レタス　カラ　ノ　カンイ　ナ　ゲノム　チュウシュツホウホウ　ノ　カクリツ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"関, 功介"},{"creatorName":"セキ, コウスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4085","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seki, Kousuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4086","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"6401_029.pdf","filesize":[{"value":"447.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"64_29","url":"https://nodai.repo.nii.ac.jp/record/786/files/6401_029.pdf"},"version_id":"11f7d9e1-44a4-479e-811c-05ed75efb75e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ゲノム抽出","subitem_subject_scheme":"Other"},{"subitem_subject":"DNAマーカー","subitem_subject_scheme":"Other"},{"subitem_subject":"ガラス繊維濾紙","subitem_subject_scheme":"Other"},{"subitem_subject":"レタス","subitem_subject_scheme":"Other"},{"subitem_subject":"ピペッティング操作","subitem_subject_scheme":"Other"},{"subitem_subject":"Genome extraction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA marker","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glass fiber filter paper","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lettuce","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pipetting operation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"DNAマーカー選抜に利用可能なレタスからの簡易なゲノム抽出方法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNAマーカー選抜に利用可能なレタスからの簡易なゲノム抽出方法の確立"},{"subitem_title":"Establishment of Simple Genomic DNA Extraction Method for Marker Assisted Selection in Lettuce","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["182"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"786","relation_version_is_last":true,"title":["DNAマーカー選抜に利用可能なレタスからの簡易なゲノム抽出方法の確立"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:44:20.089292+00:00"}