{"created":"2023-06-19T07:30:38.199914+00:00","id":587,"links":{},"metadata":{"_buckets":{"deposit":"fe6eb42b-31e2-4841-9f87-ec479cf8d57e"},"_deposit":{"created_by":3,"id":"587","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"587"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000587","sets":["47:48:119:122"]},"author_link":["3523","3524","3522","3519","3516","3517","3520","3518","3525","3521"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueNumber":"3","bibliographicPageEnd":"150","bibliographicPageStart":"144","bibliographicVolumeNumber":"60","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ユーラシアカワウソ(Lutra lutra)はユーラシア大陸に広く分布する中型食肉目である。本種には11の亜種がおり,欧州亜種L. l. lutra,東南アジア亜種L. l. barang,中国亜種L. l. chinensis などが国内外の動物園や水族館で飼育されている。欧州の動物園や水族館にはA-line(L. l. lutra)とB-line(L. l. lutraとL. l. barangが交雑した可能性がある系統)という2つのlineが存在し,日本の動物園や水族館にも導入されている。本来は同じ亜種内で繁殖がおこなわれるが,日本では本種の個体数が少ないためA-lineとB-lineで繁殖がおこなわれている。実際にB-lineが東南アジア亜種の遺伝子を持っているか評価がおこなわれ,B-lineとA-lineの間に違いがあることが示された。ただし先行研究では,解析配列が307bpと短いことが問題として上げられる。本研究ではミトコンドリア(mt)DNA Cytochrome bの全長配列(1140bp)をA-line,B-line各1個体,中国亜種2個体から決定し,先行研究で決定されたB-lineの配列を含む4配列を加えて配列比較,系統解析をおこなった。その結果,A-line,B-lineそれぞれが特徴的な変異サイトを示し,系統解析では先行研究と同じようにB-lineは中国亜種とクレイドを形成し,A-lineは欧州亜種独自のクレイドを形成した。よって解析したB-lineのmtDNAは東南アジア亜種に由来する可能性がある。2015年現在,日本のB-line個体は全て本研究で解析したB-lineの子や孫である。亜種間交雑が示唆された国内のB-lineは,本種の繁殖・維持に活用できるが,A-lineや中国亜種の系統維持に活用することはできない。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Eurasian otter (Lutra lutra) is a mid-sized species belonging to the order Carnivora and is distributed across a wide area of the Eurasian continent. This species comprises 11subspecies. The European subspecies (L. l. lutra), the Southeast Asian subspecies (L. l. barang), and the Chinese subspecies (L. l. chinensis) are reared in European and Japanese zoos and aquariums. Among these, the European facilities rear two lines, which were named A-line (L. l. lutra) and B-line (this is likely a crossbreed of L. l. lutra and L. l. barang); the Japanese facilities introduced these two lines. The mitochondrial (mt) DNA partial cytochrome b (cytb) sequence (307bp) of the A- and B-lines detected previously have shown slight but characteristic differences between the two lines. However, the previous study had limitations, as only one sample of the B-line was considered and the sequence was found to be short. Although, typically, breeding involves mating of individuals within the same subspecies, the A- and B-lines were crossbred in the Japanese facilities, which is likely because only small numbers of the Eurasian otter were reared in these facilities, thereby making cross-breeding imperative to the conservation of Eurasian otter in this country. In this study, we determined the complete cytb sequence (1140bp) from one individual each of the A- and B-lines, and two individuals of the Chinese subspecies. These sequences were compared and subjected to phylogenetic analysis, along with four individuals of Eurasian otter (which includes one B-line individual). The B-line individual showed diagnostic nucleotides at two positions, whereas the A-line individual showed diagnostic nucleotides at four positions. In the phylogenetic tree, two B-line individuals formed a monophyletic group with the two individuals of Chinese subspecies, and an A-line individual formed another monophyletic group with the other European individual. These results suggest that mtDNA sequence of B-line was derived from some Asian subspecies, and it differed from the A-line sequence (European subspecies). Moreover, because these results suggest that hybridization between the European subspecies and some Asian subspecies occurred in the B-line, the breeding program involving B-line individuals is not appropriate to the conservation of subspecies-specific strains.","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"5","subitem_description_type":"Other"}]},"item_1_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00010061300","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京農業大学大学院農学研究科バイオセラピー学専攻 "},{"subitem_text_value":" 富山市ファミリーパーク,公益社団法人日本動物園水族館協会生物多様性委員会ユーラシアカワウソ計画管理者 "},{"subitem_text_value":" 東京農業大学大学院農学研究科バイオセラピー学専攻 "},{"subitem_text_value":" ヤマザキ学園大学動物看護学部動物看護学科 "},{"subitem_text_value":" 東京農業大学大学院農学研究科バイオセラピー学専攻"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Human and Animal-Plant Relationships, Graduate School of Agriculture, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Toyama Municipal Family Park Zoo,Japanese Association of Zoos and Aquariums, Species Coordinator for Eurasian Otter "},{"subitem_text_language":"en","subitem_text_value":" Department of Human and Animal-Plant Relationships, Graduate School of Agriculture, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Faculty of Animal Health Technology, Yamazaki Gakuen University "},{"subitem_text_language":"en","subitem_text_value":" Department of Human and Animal-Plant Relationships, Graduate School of Agriculture, Tokyo University of Agriculture"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"ニホン ドウブツエン スイゾクカン キョウカイ ノ カンリ スル ユーラシア カワウソ ノ イデンテキ タヨウセイ ト ハンショク ケイカク"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"和久, 大介"},{"creatorName":"ワク, ダイスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"穴田 美佳"},{"creatorName":"アナダ, ミカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3517","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小川 博"},{"creatorName":"オガワ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3518","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安藤 元一"},{"creatorName":"アンドウ, モトカズ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3519","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木 剛"},{"creatorName":"ササキ, タケシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3520","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Daisuke, Waku","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3521","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mika Anada","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3522","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hiroshi Ogawa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3523","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motokazu Ando","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3524","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takeshi Sasaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3525","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-20"}],"displaytype":"detail","filename":"KJ00010061300.pdf","filesize":[{"value":"661.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00010061300.pdf","url":"https://nodai.repo.nii.ac.jp/record/587/files/KJ00010061300.pdf"},"version_id":"217ac6db-f204-449b-8f77-f03319cb76f6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ユーラシアカワウソ ","subitem_subject_scheme":"Other"},{"subitem_subject":" Cytochrome b 遺伝子 ","subitem_subject_scheme":"Other"},{"subitem_subject":" 亜種 ","subitem_subject_scheme":"Other"},{"subitem_subject":" 動物園 ","subitem_subject_scheme":"Other"},{"subitem_subject":" 水族館","subitem_subject_scheme":"Other"},{"subitem_subject":"Eurasian otter ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Cytochrome b gene ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Subspecies ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Zoo ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Aquarium","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"日本動物園水族館協会の管理するユーラシアカワウソの遺伝的多様性と繁殖計画","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"日本動物園水族館協会の管理するユーラシアカワウソの遺伝的多様性と繁殖計画"},{"subitem_title":"Genetic Variation and Breeding Program of Eurasian Otters in Japanese Zoos and Aquariums","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["122"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-12-18"},"publish_date":"2015-12-18","publish_status":"0","recid":"587","relation_version_is_last":true,"title":["日本動物園水族館協会の管理するユーラシアカワウソの遺伝的多様性と繁殖計画"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T07:48:01.020838+00:00"}