{"created":"2023-06-19T07:30:34.011572+00:00","id":504,"links":{},"metadata":{"_buckets":{"deposit":"b9cffdbd-0c47-4de9-b568-f00f811042b8"},"_deposit":{"created_by":3,"id":"504","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"504"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000504","sets":["47:48:109:110"]},"author_link":["2919","2923","2916","2918","2925","2922","2920","2924","2917","2921"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueNumber":"1","bibliographicPageEnd":"12","bibliographicPageStart":"6","bibliographicVolumeNumber":"58","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ポリテトラフルオロエチレン(PTFE)膜とポリプロピレン製のコニカルチューブを用いて人工容器を作製し,ニホンウズラ胚を培養した。ニホンウズラ胚は,胚盤葉期または孵卵 60 時間後から人工容器に移し,空気層の設置区・未設置区を設けた後 PTFE 膜とサランラップで封をした。また,人工容器に移す際には乳酸カルシウムを 35 mg 添加した。人工容器を用いて培養をした結果,胚盤葉期から培養した胚は気層の有無にかかわらず孵化直前である発生ステージ 45 まで徐々に生存率を下げていった。一方で,孵卵 60 時間後から培養した胚は高い生存率を維持していたが,9日目以降に空気層の未設置区では生存率が下がったのに対し設置区では高い生存率を維持していた。空気層を設置した区では,人工容器での培養開始時期に関係なく孵化直前であるステージ 45 まで到達し端打ちを行う胚が見られた。一方,空気層の未設置区では,人工容器での培養開始時期に関係なく全ての胚がステージ 45 に到達する前に死亡した。人工容器で培養し,死亡した胚を同じ培養日数または同じ発生ステージで自然発生胚と比較した結果,人工容器で培養した胚には,発生の遅れや体重,第三趾長が小さい事が分かった。また,胚盤葉期から人工容器で培養し,死亡した胚を同じ培養日数または同じ発生ステージで孵卵 60 時間後から培養した胚を比較した結果,発生の遅れや体重,第三趾長が小さい事が分かった。また,胚盤葉期から人工容器で培養した胚にのみ眼に関する奇形が見られた。そして,このことから培養初期の外胚葉へのダメージが奇形発生と培養初期の死亡率に関係があると推察された。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"An in vitro quail embryo culture system was developed using an artificial vessel, which comprised a polytetrafluoroethylene membrane and a polypropylene tube. In vitro culture was set up either directly from the blastoderm stage or after 60 hours incubation from the blastoderm stage, in the presence or absence of airspace. When embryos were cultured from the blastoderm stage, embryo viability gradually decreased in the presence of airspace from the initial stage of culture until stage 45, just before hatching. When embryos were preincubated for 60 hours before in vitro culture, viability initially remained high although mortality was high at 9 days and beyond, and all embryos died before reaching stage 45 in the absence of airspace. Using an artificial vessel under this embryo culture system, developmental stage of dead embryos was delayed, and body weight and 3rd toe length were lower than in controls (intact egg embryos). In addition, several types of malformations were observed at the blasto-derm stage, and we conjectured that early stage ectodermal damage had contributed to teratogenesis and early mortality. Moreover, the absence of airspace contributed to mortality in the later develop-mental stages.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"2","subitem_description_type":"Other"}]},"item_1_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00008527920","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京農業大学大学院農学研究科畜産学専攻 "},{"subitem_text_value":" 東京農業大学農学部バイオセラピー学科 "},{"subitem_text_value":" 東京農業大学農学部バイオセラピー学科 "},{"subitem_text_value":" 東京農業大学農学部畜産学科客員教授 "},{"subitem_text_value":" 東京農業大学農学部バイオセラピー学科"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Animal science, Graduate School of Agriculture, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Human and Animal-Plant Relationships, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Human and Animal-Plant Relationships, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Affiliate professor, Department of Animal Science, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Human and Animal-Plant Relationships, Tokyo University of Agriculture"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"ジンコウ ヨウキ バイヨウ ガ ウズラハイ ノ ハッセイ ニ オヨボス エイキョウ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福永, 一朗"},{"creatorName":"フクナガ, イチロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木 剛"},{"creatorName":"ササキ, タケシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安藤 元一"},{"creatorName":"アンドウ, モトカズ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2918","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"橋本 光一郎"},{"creatorName":"ハシモト, コウイチロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2919","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小川 博"},{"creatorName":"オガワ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2920","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukunaga, Ichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2921","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasaki Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2922","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ando Motokazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2923","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hashimoto Koichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2924","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2925","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-20"}],"displaytype":"detail","filename":"KJ00008527920.pdf","filesize":[{"value":"714.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00008527920.pdf","url":"https://nodai.repo.nii.ac.jp/record/504/files/KJ00008527920.pdf"},"version_id":"2911f009-e375-43f6-92ac-af51fd3f63f8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"人工容器 ","subitem_subject_scheme":"Other"},{"subitem_subject":" 鳥類胚培養 ","subitem_subject_scheme":"Other"},{"subitem_subject":" 奇形 ","subitem_subject_scheme":"Other"},{"subitem_subject":" ニホンウズラ","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro culture system ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" avian embryo culture ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" malformations ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Japanese quail","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"人工容器培養がウズラ胚の発生におよぼす影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"人工容器培養がウズラ胚の発生におよぼす影響"},{"subitem_title":"Influence of In vitro Culture System on Development of Quail Embryo","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["110"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-14"},"publish_date":"2013-06-14","publish_status":"0","recid":"504","relation_version_is_last":true,"title":["人工容器培養がウズラ胚の発生におよぼす影響"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T07:50:01.138473+00:00"}