{"created":"2023-06-19T07:30:33.759021+00:00","id":498,"links":{},"metadata":{"_buckets":{"deposit":"8603a0a4-ccc1-4c5f-b09a-0ae59bafb2cf"},"_deposit":{"created_by":3,"id":"498","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"498"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000498","sets":["47:48:104:108"]},"author_link":["2869","2873","2867","2871","2872","2868","2866","2864","2865","2870"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueNumber":"4","bibliographicPageEnd":"266","bibliographicPageStart":"261","bibliographicVolumeNumber":"57","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"RAPD および ISSR 法により,シクラメン(Cyclamen)属内における種および個体間の遺伝的変異を評価し,類縁関係を推定した。シクラメン属野生種(C. persicum, C. coum, C.purpurascens, C. africanum, C. hederifolium, C. graecum およびC. rohlfsianum)および園芸品種(C. persicum cv.カールソ,ネオゴールデンガール)の計 7種 24個体について,17種のランダムプライマーおよび 11種の ISSR プライマーを用いた多型解析の結果,453 のバンドが検出され,全てのバンドで多型がみられた。そのうち,55 のバンドは種特異的であった。多型データに基づく AMOVA 解析の結果,遺伝的変異は種内と比べて種間で大きく,種の分化が明確に示された。同データによる数量化理論III類より 7種は 4つのグループに分けられた。そこで,野生種のみのデータを用いた UPGMA 法によるクラスター解析を行った結果,7種は 3つのクラスターを構成し,シクラメン属内の亜属を Psilanthum, Gryophoebe, Cyclamen および Eucosme とする,既報の分類仮説と一致した。本研究は,RAPD および ISSR マーカーを用いてシクラメン属内の類縁関係を示した最初の報告であり,これらマーカーによるシクラメン種の識別の可能性を示唆した。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) techniques were employed to estimate the genetic variation and relationship among and within species in seven species of Cyclamen. 453 bands were scored by 17 random primers and 11 ISSR primers used for analysis, with all bands (100%) being polymorphic and 55 bands specific for a species. Based on the polymorphic data, the among-species variation was larger than the within-species variation. Quantification theory (Suryoka) III showed that seven species were divided into four separate groups. Furthermore, unweighted pair-group method with arithmetic mean (UPGMA) analysis based on the data of only wild species formed three subclusters, which agreed with one of the hypotheses classified within Cyclamen genus. Our results indicate, for the first time, the genetic relationships within Cyclamen genus following the application of a RARD and ISSR marker, and the efficacy of RAPD and ISSR markers to distinguish species in Cyclamen.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"4","subitem_description_type":"Other"}]},"item_1_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00008376279","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京農業大学短期大学部生物生産技術学科 "},{"subitem_text_value":" 東京農業大学農学部農学科 "},{"subitem_text_value":" 東京農業大学短期大学部生物生産技術学科 "},{"subitem_text_value":" 東京農業大学農学部農学科 "},{"subitem_text_value":" 東京農業大学名誉教授"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Bioproduction Technology, Junior College of Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Agriculture, Faculty of Agriculture, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Bioproduction Technology, Junior College of Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Agriculture, Faculty of Agriculture, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Professor Emeritus, Tokyo University of Agriculture"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"Cyclamen spp. ノ RAPD オヨビ ISSR マーカー ニ ヨル ルイエン カンケイ ノ ヒョウカ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"和久井, 健司"},{"creatorName":"ワクイ, ケンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2864","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡部 慎也"},{"creatorName":"オカベ, シンヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2865","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小松 憲治"},{"creatorName":"コマツ, ケンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2866","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"丹羽 克昌"},{"creatorName":"ニワ, カツマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2867","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤垣 順三"},{"creatorName":"フジガキ, ジュンゾウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2868","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakui, Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okabe Shinya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Komatsu Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Niwa Katsumasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2872","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujigaki Junzo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2873","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-20"}],"displaytype":"detail","filename":"KJ00008376279.pdf","filesize":[{"value":"784.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00008376279.pdf","url":"https://nodai.repo.nii.ac.jp/record/498/files/KJ00008376279.pdf"},"version_id":"92b8e005-e051-48d2-b783-a3e9906aacb2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cyclamen属 ","subitem_subject_scheme":"Other"},{"subitem_subject":" Random amplified polymorphic DNA(RAPD) ","subitem_subject_scheme":"Other"},{"subitem_subject":" Inter simple sequence repeat (ISSR)","subitem_subject_scheme":"Other"},{"subitem_subject":"Cyclamen ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Random amplified polymorphic DNA (RAPD) ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Inter simple sequence repeat (ISSR)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cyclamen spp. の RAPD および ISSR マーカーによる類縁関係の評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cyclamen spp. の RAPD および ISSR マーカーによる類縁関係の評価"},{"subitem_title":"Genetic Relationship Based on RAPD and ISSR Analyses in Cyclamen ssp.","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["108"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-03-18"},"publish_date":"2013-03-18","publish_status":"0","recid":"498","relation_version_is_last":true,"title":["Cyclamen spp. の RAPD および ISSR マーカーによる類縁関係の評価"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T07:50:27.130223+00:00"}