@article{oai:nodai.repo.nii.ac.jp:00000327,
 author = {馬場, 正 and 石川 一憲 and 池田富喜夫 and BABA, Tadashi and ISHIKAWA Kazunori and IKEDA Fukio},
 issue = {2},
 month = {2016-04-20},
 note = {ブドウ4倍性品種の安定した無核果生産に,ストレプトマイシン(SM)の利用が進められている。本研究では,4倍性品種`藤稔'を用い,SMの無核化作用を明らかにしようと試みた。200ppmのSMもしくは25ppmのジベレリン(GA)を満開約2週間前に処理し,無核果率,花粉の発芽率,胚のう発育を調査した。調査した2ヵ年とも,SM処理区で最も高い無核化効果を示した。花粉の発芽率はGAまたはSM処理により低下したが,受精を妨げるほどではなかった。そこで胚のう発育への影響を観察したところ,SMは満開日までの胚のう,とくに卵装置の発育を遅らせており,さらに引き続いて起こる胚乳核の分裂を停止ないし遅延させていた。一方GAは,卵装置の発育を妨げていなかった。GAまたはSMの処理適期を検討したところ,GAが満開33日前から満開15日後までのどの時期に処理しても比較的高い無核化効果を示したのに対し,SMは満開23日前から8日前の胚のう母細胞が減数分裂する時期が処理適期であった。このようにSMは胚のう発育に対し明らかに阻害的に働いており,GAによる無核化作用とは異なることが示唆された。, Streptomycin (SM) has been adopted to induce seedlessness among tetraploid grape varieties. This study was conducted on tetraploid grape 'Fujiminori' to gain a better understanding of the mechanism of seedlessness induction by SM. Seedlessness, pollen germination and embryo sac development were investigated in clusters dipped in SM 200ppm and gibberellic acid (GA) 25ppm solutions. SM treatment about two weeks before full bloom resulted in the highest level of seedlessness during both years of the investigation. At full bloom, the degeneration of embryo sac and especially the retarding of egg apparatus development were observed more frequently in the berries treated with SM while inhibition of pollen germination was less significant. GA did not affect egg apparatus development. Effect of GA on seedlessness was constant from 33 days before full bloom to 15 days after full bloom. Effect of SM was limited to a period from 23 days to 8 days before full bloom when meiotic division of embryo sac mother cell was occurring. These data suggest that induction of seedlessness by SM can be attributed to inhibition of embryo sac development, and is an alternative mechanism compared with the effects of GA., E, 7, KJ00005049281, 論文, Article},
 pages = {139--143},
 title = {ブドウa藤稔aの胚のう発育に対するストレプトマイシンの阻害効果},
 volume = {53},
 year = {},
 yomi = {ババ, タダシ and イシカワ, カズノリ and イケダ, フキオ}
}