{"created":"2023-06-19T07:30:23.670261+00:00","id":292,"links":{},"metadata":{"_buckets":{"deposit":"e25b216a-01db-4ab5-aa50-43b6cce32595"},"_deposit":{"created_by":3,"id":"292","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"292"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000292","sets":["47:48:79:81"]},"author_link":["1437","1430","1436","1429","1433","1434","1425","1432","1424","1435","1428","1427","1431","1426"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueNumber":"2","bibliographicPageEnd":"114","bibliographicPageStart":"109","bibliographicVolumeNumber":"52","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ジャガイモYウイルス(PVY)はシリアのジャガイモ生産における主要ウイルスの一つとされ,ジャガイモ生産の阻害要因となっている。PVY感染の無い種イモの生産と供給が主要な防除手段であるが,そのためには,効果的なウイルス検査法が必要である。酵素結合抗体法(ELISA)は複数種類のジャガイモウイルス検出には最も一般的な技術であるが,それぞれのウイルスに対して特異的な抗血清が利用できることが必須条件となる。本研究では,シリアに発生したPVY分離株を純化し,ウサギに免疫することにより抗血清をはじめて作製した。この抗血清を利用し多くのシリア産PVYを用いたELISAによる検討では,市販の抗血清と同等の検出能力を有し,両抗血清間に結果の相違は認められなかった。また,非特異的反応も生じなかった。さらに,検出限界は高かった。以上より,本研究ではシリアで分離されたPVYに対して初めてシリア国産抗血清を得ることができ,抗血清の恒常的輸入を不要にしたことに大きな意義があると考えられる。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Potato virus Y (PVY) is reported to be the main virus occurring in potato fields and it increases the cost of potato production in Syria. Producing and utilizing of certified seed potatoes with minimum PVY infection is the main control key that requires efficient virus detection methods. Enzyme linked immunosorbent assay (ELISA) using specific antisera is the most common method for the detection of potato viruses. In this study we report the production of PVY antiserum in Syria for the first time. This antibody was produced against a Syrian PVY isolate and its reliability was confirmed by testing a panel of PVY infected samples collected from Syria. The results of ELISA using this antibody were consistent with those obtained using Agdia PVY antibody and no significant differences were found. In addition this antibody was highly specific to PVY with undetectable cross reaction to plant proteins. Moreover it was shown to be very sensitive and PVY infection could be detected in highly diluted plant saps. Since this antibody was produced against a Syrian isolate of PVY it has advantages over imported PVY specific antiserum.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"5","subitem_description_type":"Other"}]},"item_1_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00004735039","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"シリア国,Ministry of Agriculture and Agrarian Reform, General Organization for Seed Multiplication(GOSM) "},{"subitem_text_value":" シリア国,Ministry of Agriculture and Agrarian Reform, General Organization for Seed Multiplication(GOSM),東京農業大学農学研究科国際農業開発学 "},{"subitem_text_value":" 国際協力事業団 "},{"subitem_text_value":" 独立行政法人種苗管理センター "},{"subitem_text_value":" シリア国,Ministry of Agriculture and Agrarian Reform, General Organization for Seed Multiplication(GOSM) "},{"subitem_text_value":" シリア国,Ministry of Agriculture and Agrarian Reform, General Organization for Seed Multiplication(GOSM) "},{"subitem_text_value":" 東京農業大学農学研究科国際農業開発学"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"General Organization for Seed Multiplication (GOSM), Ministry of Agriculture and Agrarian Reform, Syria "},{"subitem_text_language":"en","subitem_text_value":" General Organization for Seed Multiplication (GOSM), Ministry of Agriculture and Agrarian Reform, Syria,Department of International Agricultural Development, Graduate School of Agriculture, Tokyo University of Agriculture, Japan "},{"subitem_text_language":"en","subitem_text_value":" Japan International Cooperation Agency (JICA), Japan "},{"subitem_text_language":"en","subitem_text_value":" National Center for Seeds and Seedlings, Japan "},{"subitem_text_language":"en","subitem_text_value":" General Organization for Seed Multiplication (GOSM), Ministry of Agriculture and Agrarian Reform, Syria "},{"subitem_text_language":"en","subitem_text_value":" General Organization for Seed Multiplication (GOSM), Ministry of Agriculture and Agrarian Reform, Syria "},{"subitem_text_language":"en","subitem_text_value":" Department of International Agricultural Development, Graduate School of Agriculture, Tokyo University of Agriculture, Japan"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"シリアサンジャガイモYウイルス ニ タイスル ポリクローナルコウタイ ノ ショサクセ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sankari, Shadi"},{"creatorName":"Sankari, Shadi","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1424","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chikh Ali Mohamad"},{"creatorName":"Chikh, Ali Mohamad","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1425","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"片山 克己"},{"creatorName":"カタヤマ, カツミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1426","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三木 信雄"},{"creatorName":"ミキ, ノブオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1427","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Said Omar Abdul Mohasen"},{"creatorName":"Said, Omar Abdul Mohasen","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1428","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sawas Ahmad Bahij"},{"creatorName":"Sawas, Ahmad Bahij","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1429","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"夏秋啓子"},{"creatorName":"ナツアキ, ケイコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1430","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sankari, Shadi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1431","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chikh Ali Mohamad","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1432","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katayama Katsumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1433","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miki Nobuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1434","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Said Omar Abdul Mohasen","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1435","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sawas Ahmad Bahij","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1436","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Natsuaki Keiko T.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1437","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-20"}],"displaytype":"detail","filename":"KJ00004735039.pdf","filesize":[{"value":"789.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004735039.pdf","url":"https://nodai.repo.nii.ac.jp/record/292/files/KJ00004735039.pdf"},"version_id":"0cbb542c-4c90-4b03-93cb-5c4b04ed0482"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"種ジャガイモ ","subitem_subject_scheme":"Other"},{"subitem_subject":" ELISA ","subitem_subject_scheme":"Other"},{"subitem_subject":" ジャガイモYウイルス ","subitem_subject_scheme":"Other"},{"subitem_subject":" 抗血清 ","subitem_subject_scheme":"Other"},{"subitem_subject":" シリア","subitem_subject_scheme":"Other"},{"subitem_subject":"Seed potatoes ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" ELISA ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Potato virus Y ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Antibody ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Syria","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"シリア産ジャガイモYウイルスに対するポリクローナル抗体の初作製","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シリア産ジャガイモYウイルスに対するポリクローナル抗体の初作製"},{"subitem_title":"The First Report of Polyclonal Antibody Production of a Syrian Isolate of Potato virus Y","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["81"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-09-20"},"publish_date":"2007-09-20","publish_status":"0","recid":"292","relation_version_is_last":true,"title":["シリア産ジャガイモYウイルスに対するポリクローナル抗体の初作製"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T07:56:23.990132+00:00"}