{"created":"2023-06-19T07:30:19.860221+00:00","id":222,"links":{},"metadata":{"_buckets":{"deposit":"8337158c-57d8-407d-8f99-379732e73aad"},"_deposit":{"created_by":3,"id":"222","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"222"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000222","sets":["47:48:64:67"]},"author_link":["982","981","984","983","980","985"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueNumber":"3","bibliographicPageEnd":"89","bibliographicPageStart":"82","bibliographicVolumeNumber":"49","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"感受性高槻系,抵抗性八千代系イエバエ腹部グルタチオンS-転移酵素(GST)分子種6A,6Bの遺伝子をクローニングし大腸菌内で発現させた。イエバエ腹部GST分子種6Bは,6Aと同様にプロチオホスオキソンに対してグルタチオン(GSH)のアルキル抱合を担うが,6Aと異なるのは抵抗性由来のGST/6Bの方が感受性由来のGST6Bより脱エチル体の生成量が約12と少なく,解毒化への抱合反応を抑制していた。また,in vitroの酸化的GSH抱合反応はGST6Bが新奇なGSH抱合反応を少なくとも一つ担うことを示唆した。プロチオホスオキソンS-オキシドのGSHとの反応をBioMedCACheを用いた計算化学において予測した。一つはGS^-のリン原子へのSN2反応,もう一つはGS^-のエトキシル基のメチレン炭素原子へのSN2反応であった。十分な証拠に欠けるがGST6Bの存在がイエバエの抵抗性発現を抑制するのに重要な役割を演じているかもしれない。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The cloning of glutathione S-transferase (GST) 6A and 6B genes in the susceptible and resistant houseflies (Takatsuki and Yachiyo strains) and the expression of their genes in Escherichia coli were carried out to obtain the GST6A and 6B enzymes. Similarly to the housefly abdomen GST6A enzymes, the housefly abdomen GST6B enzymes conjugated alkyl group on phosphorus atom of prothiophos oxon with glutathione. However, the GST6B enzyme derived from the resistant housefly had only 1/2 level of GST activity for the above GSH conjugation reaction, compared with the GST6B derived from the susceptible housefly, to decrease the ability in detoxification. On the other hand, in vitro oxidative GSH conjugation reaction indicated that their GST6B enzymes cover a novel GSH conjugation reaction at least. The reaction of prothiohos oxon S-oxide with GSH was predicted on computational chemistry using BioMedCAChe software. One was SN2 reaction of GS^- to phosphorus atom, and the other was SN2 reacion of GS^- to methylene carbon atom of ethoxyl group. The GST6B enzyme probably plays an important role in the control of resistant decrease for houseflies.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"3","subitem_description_type":"Other"}]},"item_1_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00003960123","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Articles","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京農業大学農学研究科農芸化学 "},{"subitem_text_value":" 東京農業大学応用生物科学部生物応用化学科 "},{"subitem_text_value":" 東京農業大学応用生物科学部生物応用化学科"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Appllied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Department of Appllied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"ユウキリン サッチュウザイ プロチオホス ノ イエバエ フクブ グルタチオン S-テンイ コウソ 6B ニ ヨル シンキ ホウゴウ ハンノウ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"箕川, 剛"},{"creatorName":"ミカワ, ツヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"980","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須恵 雅之"},{"creatorName":"スエ, マサユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"981","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本 徹"},{"creatorName":"ミヤモト, トオル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"982","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mikawa, Tsuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"983","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sue  Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"984","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamoto  Toru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"985","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-20"}],"displaytype":"detail","filename":"KJ00003960123.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00003960123.pdf","url":"https://nodai.repo.nii.ac.jp/record/222/files/KJ00003960123.pdf"},"version_id":"3714d855-b7ee-4cfe-9218-843273421db5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"有機リン殺虫剤プロチオホス ","subitem_subject_scheme":"Other"},{"subitem_subject":" イエバエグルタチオンS-転移酵素6B ","subitem_subject_scheme":"Other"},{"subitem_subject":" 抵抗性","subitem_subject_scheme":"Other"},{"subitem_subject":"Organophosphorus Insecticide Prothiophos ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Housefly glutathione S-transferase 6B iso-zyme ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"有機リン殺虫剤プロチオホスのイエバエ腹部グルタチオンS-転移酵素6Bによる新奇抱合反応","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"有機リン殺虫剤プロチオホスのイエバエ腹部グルタチオンS-転移酵素6Bによる新奇抱合反応"},{"subitem_title":"A Novel Glutathione Conjugation of the Organophosphorus Insecticide Prothiophos by the Housefly Abdomen Glutathione S-transferase 6B","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["67"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-12-01"},"publish_date":"2004-12-01","publish_status":"0","recid":"222","relation_version_is_last":true,"title":["有機リン殺虫剤プロチオホスのイエバエ腹部グルタチオンS-転移酵素6Bによる新奇抱合反応"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T07:58:20.814047+00:00"}