@article{oai:nodai.repo.nii.ac.jp:00000222,
 author = {箕川, 剛 and 須恵 雅之 and 宮本 徹 and Mikawa, Tsuyoshi and Sue  Masayuki and Miyamoto  Toru},
 issue = {3},
 month = {2016-04-20},
 note = {感受性高槻系,抵抗性八千代系イエバエ腹部グルタチオンS-転移酵素(GST)分子種6A,6Bの遺伝子をクローニングし大腸菌内で発現させた。イエバエ腹部GST分子種6Bは,6Aと同様にプロチオホスオキソンに対してグルタチオン(GSH)のアルキル抱合を担うが,6Aと異なるのは抵抗性由来のGST/6Bの方が感受性由来のGST6Bより脱エチル体の生成量が約12と少なく,解毒化への抱合反応を抑制していた。また,in vitroの酸化的GSH抱合反応はGST6Bが新奇なGSH抱合反応を少なくとも一つ担うことを示唆した。プロチオホスオキソンS-オキシドのGSHとの反応をBioMedCACheを用いた計算化学において予測した。一つはGS^-のリン原子へのSN2反応,もう一つはGS^-のエトキシル基のメチレン炭素原子へのSN2反応であった。十分な証拠に欠けるがGST6Bの存在がイエバエの抵抗性発現を抑制するのに重要な役割を演じているかもしれない。, The cloning of glutathione S-transferase (GST) 6A and 6B genes in the susceptible and resistant houseflies (Takatsuki and Yachiyo strains) and the expression of their genes in Escherichia coli were carried out to obtain the GST6A and 6B enzymes. Similarly to the housefly abdomen GST6A enzymes, the housefly abdomen GST6B enzymes conjugated alkyl group on phosphorus atom of prothiophos oxon with glutathione. However, the GST6B enzyme derived from the resistant housefly had only 1/2 level of GST activity for the above GSH conjugation reaction, compared with the GST6B derived from the susceptible housefly, to decrease the ability in detoxification. On the other hand, in vitro oxidative GSH conjugation reaction indicated that their GST6B enzymes cover a novel GSH conjugation reaction at least. The reaction of prothiohos oxon S-oxide with GSH was predicted on computational chemistry using BioMedCAChe software. One was SN2 reaction of GS^- to phosphorus atom, and the other was SN2 reacion of GS^- to methylene carbon atom of ethoxyl group. The GST6B enzyme probably plays an important role in the control of resistant decrease for houseflies., E, 3, KJ00003960123, 論文, Articles},
 pages = {82--89},
 title = {有機リン殺虫剤プロチオホスのイエバエ腹部グルタチオンS-転移酵素6Bによる新奇抱合反応},
 volume = {49},
 year = {},
 yomi = {ミカワ, ツヨシ and スエ, マサユキ and ミヤモト, トオル}
}