{"created":"2023-06-19T07:30:19.816946+00:00","id":221,"links":{},"metadata":{"_buckets":{"deposit":"69e52c07-beda-4092-9c5c-e4fb47ec24a8"},"_deposit":{"created_by":3,"id":"221","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"221"},"status":"published"},"_oai":{"id":"oai:nodai.repo.nii.ac.jp:00000221","sets":["47:48:64:67"]},"author_link":["977","976","975","974","978","979"],"item_1_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueNumber":"3","bibliographicPageEnd":"81","bibliographicPageStart":"75","bibliographicVolumeNumber":"49","bibliographic_titles":[{}]}]},"item_1_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"バナナ(Musa spp)にとって,ウイルスの存在は,生産の場だけではなく,遺伝資源としての種や品種の保存においても重大な脅威である。本研究では,インドネシアのジョグジャカルタ特別州に位置するバナナ品種保存園において,38品種68株のバナナを3年間に渡って採集し,血清学的・分子生物学的手法の両方または一方を用いて,ウイルスの検出を行った。その結果,キュウリモザイクウイルス(CMV)は3株のみで感染が確認されたのに対し,バナナバンチートップウイルス(BBTV)は21株で認められたことから,本来はウイルスフリーであるべき本品種保存園においてもBBTVは蔓延していることが示された。また,調査を行った38品種は長期間同じ環境条件下で栽培されていたが,BBTVは12品種で,CMVは3品種でそれぞれ病徴を伴って感染が確認された。このことから,これらの12品種と3品種はそれぞれBBTVとCMVに対して,より感受性であると思われた。バナナ品種保存園に発生したBBTV2分離株(BBTV-IG33, -IG64)とその近郊の農村に発生したBBTV1分離株(BBTV-IJs11)についてDNA-1とDNA-3の塩基配列を決定した結果,それぞれのコンポーネントの全長で3分離株は高い相同性を有していた(98～99%,99～100%)。また,DNA-1のCR-M領域(major common region)では,既報のオーストラリアおよびフィジーの分離株よりも,日本・台湾・フィリピン・中国・ベトナムの分離株と,より高い値を示した(63～65%,93～95%)。したがって,インドネシアにおけるBBTVアジアグループ(KARANら,1994)の発生とそれらの分子生物学的性状が本研究によって初めて明確に示された。","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"For banana plants (Musa spp.), occurrence of viruses is serious threat not only for production but also in conservation of banana species/cultivars as genetic resources. In this study, we collected 68samples of 38banana cultivars during 3years of survey and carried out the detection of viruses by serological and/or molecular methods in a banana germplasm garden of central Java, Indonesia. Banana bunchy top virus (BBTV) and Cucumber mosaic virus (CMV) were confirmed from 21samples and 3samples, respectively, though Banana bract mosaic virus (BBrMV) was not detected in this survey. Thus it was made clear that the major constraint virus is BBTV in this germplasm garden where it should be free from any virus infection. The occurrences of BBTV were confirmed in 12cultivars and of CMV were confirmed in 3cultivars among 38cultivars along with viral symptoms though all of tested cultivars were grown under the same condition for long time. Based upon this results, it was supposed that these 12 and 3cultivars are more sensitive to BBTV and CMV infection, respectively. Two BBTV isolates (BBTV-IG33 and IG64) in this germplasm garden and one isolate of BBTV (BBTV-IJs11) from a village nearby the germplasm garden were sequenced for their DNA-1 and DNA-3 and shown to have high homologies among them in full-length of each component (98 to 99% and 99 to 100%, respectively). In major common region (CR-M) of DNA-1, the Indonesian isolates showed higher homology obviously with isolates from Japan, Taiwan, the Philippines, China and Vietnam than with isolates from Australia and Fiji (93 to 95% and 63 to 65%, respectively). Therefore, molecular characters of these Indonesian BBTV isolates, which were classified into the Asian group (KARAN et al., 1994), were firstly reported and analyzed in this study.","subitem_description_type":"Other"}]},"item_1_description_13":{"attribute_name":"レポート・講演番号","attribute_value_mlt":[{"subitem_description":"E","subitem_description_type":"Other"}]},"item_1_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"2","subitem_description_type":"Other"}]},"item_1_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00003960122","subitem_description_type":"Other"}]},"item_1_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Articles","subitem_description_type":"Other"}]},"item_1_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00164184","subitem_source_identifier_type":"NCID"}]},"item_1_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京農業大学農学研究科国際農業開発学 "},{"subitem_text_value":" インドネシア国立ガジャマダ大学農学部 "},{"subitem_text_value":" 東京農業大学国際食料情報学部国際農業開発学科"}]},"item_1_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of international Agricultural Development, Graduate School of Agriculture, Tokyo University of Agriculture "},{"subitem_text_language":"en","subitem_text_value":" Faculty of Agriculture, Gadjah Mada University "},{"subitem_text_language":"en","subitem_text_value":" Department of International Agricultural Development, Faculty of International Agriculture and Food Studies, Tokyo University of Agriculture"}]},"item_1_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"インドネシア ニ オケル バナナ ガイライシュ カラ ノ ウイルス ケンシュツ オヨビ バナナ バンチートップ ウイルス ノ ブンシ セイブツガク テキ ショ カイセキ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"古屋, 典子"},{"creatorName":"フルヤ, ノリコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"974","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Somowiyarjo  Susamto"}],"nameIdentifiers":[{"nameIdentifier":"975","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"夏秋 啓子"},{"creatorName":"ナツアキ, ケイコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"976","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furuya, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"977","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Somowiyarjo  Susamto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"978","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Natsuaki  T. Keiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"979","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-20"}],"displaytype":"detail","filename":"KJ00003960122.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00003960122.pdf","url":"https://nodai.repo.nii.ac.jp/record/221/files/KJ00003960122.pdf"},"version_id":"1a2ab6d2-1650-4781-bdba-d7900525d6d7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ウイルス検出 ","subitem_subject_scheme":"Other"},{"subitem_subject":" インドネシア ","subitem_subject_scheme":"Other"},{"subitem_subject":" バナナ品種 ","subitem_subject_scheme":"Other"},{"subitem_subject":" バナナバンチートップウイルス ","subitem_subject_scheme":"Other"},{"subitem_subject":" 塩基配列","subitem_subject_scheme":"Other"},{"subitem_subject":"virus detection ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Indonesia ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" banana cultivar ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" Banana bunchy top virus ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":" nucleotide sequence","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"インドネシアにおけるバナナ在来種からのウイルス検出およびバナナバンチートップウイルスの分子生物学的初解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"インドネシアにおけるバナナ在来種からのウイルス検出およびバナナバンチートップウイルスの分子生物学的初解析"},{"subitem_title":"Virus Detection from Local Banana Cultivars and the First Molecular Characterization of Banana bunchy top virus in Indonesia","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["67"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-12-01"},"publish_date":"2004-12-01","publish_status":"0","recid":"221","relation_version_is_last":true,"title":["インドネシアにおけるバナナ在来種からのウイルス検出およびバナナバンチートップウイルスの分子生物学的初解析"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T07:58:20.031049+00:00"}