@article{oai:nodai.repo.nii.ac.jp:00000188,
 author = {和田, 健太 and 早川 千里 and 横濱 道成 and Wada, Kenta and Hayakawa Chisato and Yokohama Michinari},
 issue = {4},
 month = {2016-04-20},
 note = {エゾシカ(Cervus nippon yesoensis)を有効に活用・保存するためには,基礎的な遺伝的情報を蓄積する必要がある。我々は北海道産エゾシカのミトコンドリアDNA(mtDNA)の12SrRNA遺伝子(957bp),COX II遺伝子(687bp)およびD-loop領域(1,110bp)のDNA配列を解析し,他のシカ科(サンバー,Cervus unicolor ; アカシカ,Cervus elaphus ; タイワンキョン,Muntiacus reevesi ; オジロジカ,Odocoi-leus virginianus ; オグロジカ,Odocoileus hemionus他)動物との比較を行った。12SrRNA遺伝子について解析したところ,調べたCervus属3種はクラスターを形成し,エゾシカはサンバーおよびアカシカに対してそれぞれ2.9および3.5%の塩基置換度を示した。系統樹では,エゾシカとサンバーが最も近く,次いでアカシカが結合した。そのCervus属にMuntiacus属が結合し,Odocoileus属よりも近い関係にあった。COX II遺伝子の解析から,エゾシカとサンバーとの塩基置換度は4.2%であり,またエゾシカとオジロジカとは11.8%であった。系統樹では,12SrRNA遺伝子と同様にエゾシカとサンバーが最も近く,次いでオジロジカが結合した。D-loop領域の解析から,エゾシカと岩手県産のホンシュウジカ(C.n. centralis)との塩基置換度は5.2%と低い値を示した。しかし,山口県産ホンシュウジカおよびツシマジカ(C.n. pulchellus対馬列島産)における塩基置換度は岩手県産のホンシュウジカに比べて高かった(16.6～16.8%)。系統樹では,本邦におけるニホンジカが南北の両グループに分かれ,アジアのニホンジカ,アカシカおよびサンバーは,南グループのニホンジカに近い関係にあった。, Accumulation of genetic information is important in order to efficiently utilize and preserve the Yeso Sika deer (Cervus nippon yesoensis). We analyzed DNA sequences of the 12SrRNA gene, Cytochrome c Oxidase II (COX II) gene and the D-loop region of the mitochondrial DNA in the Yeso Sika deer. The sequence divergence of mtDNA was compared in pairs between other Cervidae (Cervus unicolor, Cervus elaphus, Muntiacus reevesi, Odocoileus virginianus, Odocoileus hemionus, etc ). Moreover, phylogenetic analysis was also done between them. The complete sequence of the 12SrRNA gene of the Yeso Sika deer was 957bp, and the sequence divergence between the Yeso Sika deer and Cervus unicolor (Samber) or Cervus elaphus (Red deer) was low in value at 2.9 and 3.5%, respectively. In the phylogenetic tree, the Yeso Sika deer and the Samber were joined first, and following the Red deer was connected to them. Finally, Muntiacus reevesi (Reeves'muntjac) was connected to the genus Cervus. As a result of the phylogenetic analysis, we see that the Reeves' muntjac had a closer relationship to the genus Cervus than the genus Odocoileus. The complete sequence of the COX II gene in the Yeso Sika deer was 687bp, and the sequence divergence between the Yeso Sika deer and the Samber or Odocoileus virginianus (White-tailed deer) was 4.2 and 11.8%, respectively. In the phylogenetic tree of the COX II gene, the Yeso Sika deer and the Samber were most closely related as well as the 12SrRNA gene. The next closest relationship was of the White-tailed deer and the genus Cervus. The D-loop region of the Yeso Sika deer was 1110bp, and the sequence divergence between the Yeso Sika deer and the Cervus nippon centralis/Gyo1 (from Iwate Prefecture-Honsyu Sika deer) showed the lowest value (5.2%). Furthermore, the sequence divergence of the Honsyu Sika deer/Yma1 (from Yamaguchi Prefecture) and the C.n. pulchellus (Tushima Sika deer ) was high in value (16.6-16.8%). In the phylogenetic tree, the Sika deer in Japan was classified into two groups of North and South. In addition, the Sika deer of Asia, the Red deer and the Samber were close in relationship to the South group of the Japanease Sika deer., E, 8, KJ00004074207, 論文, Article},
 pages = {298--312},
 title = {エゾシカ(Cervus nippon yesoensis)のミトコンドリアDNA塩基配列の解析-12SrRNA遺伝子,COX II遺伝子およびD-loop領域-},
 volume = {47},
 year = {},
 yomi = {ワダ, ケンタ and ハヤカワ, チサト and ヨコハマ, ミチナリ}
}